Figure 2.
Tβ4 induces transcription of PAI-1 promoter in EA.hy 926 cells. GFP expression in cultured EA.hy 926 cells was analyzed after transfection with pGFP-PAI-1 reporter constructs. EA.hy 926 cells grown in slide chambers at a density of 2 × 105cells/mL were transfected with 1 μg pGFP-PAI-1 using LipofectAMINE and incubated with 0, 40, and 80 nM Tβ4 for 24 hours. The top panels are fluorescent images, and the bottom panels are the corresponding phase-contrast micrographs. This experiment has been repeated 3 times with similar results.
