Effect of plasma on megakaryocyte ploidy distribution. Histograms show the cell ploidy distribution of CD34⁺-rich cells cultured for 10 days in the following plasmas (left to right): control, ITP-1, ITP-5 and ITP-18. Cells cultured in control or ITP-18 plasma (no evident suppression of in vitro megakaryocyte production) show 4 distinct peaks, 2N, 4N, 8N, 16N, while cells cultured in ITP-1 or ITP-5 plasma (both suppressed in vitro megakaryocyte production) show primarily 2N cells.