Structure and tissue expression of EBP. (A) Schematic representation of EBP. EBP encodes a protein of 767 amino acids and comprises a central proline-rich region (PRR) followed by 4 SH3 domains. The SH3 domains are indicated by black boxes, and the PRR is shown as hatched boxes. Clone nos. 30 and 46 were obtained from yeast 2-hybrid library screening. Reported sequence DKFZp434D0215 (GI: 27477709) that shows nucleotide sequence identity with EBP is aligned for comparison. (B) Deduced amino acid sequence of EBP. The PRR within amino acids 320-350 is boldfaced. Putative EEN-binding motif, PPERP in amino acids 343-347, is marked by asterisks. Four SH3 domains located in amino acids 473-529, 552-606, 639-696, and 708-765 are underlined. (C) Tissue expression of EBP. Analysis of EBP expression by PCR in multiple human adult (1-8) and fetal (9-16) tissue cDNAs. Arrowheads indicate specific PCR products amplified. PCR of actin is included as control. Heart (1,9), brain (2,10), placenta (3), lung (4,11), liver (5,12), skeletal muscle (6,13), kidney (7,14), pancreas (8), spleen (15), thymus (16), water (17), EBP plasmid DNA (18), 100-bp ladder marker (M).