Phosphorylation of AKT in CLL cells isolated from patients who have received intravenous Hu1D10 for the treatment of CLL. In conjunction with a clinical trial of Hu1D10, blood samples were obtained at pretreatment and 10, 30, and 120 minutes following the infusion of Hu1D10. Following density gradient isolation of lymphocytes, whole cell lysates were made and analyzed using immunoblots to determine relative changes in phosphorylated AKT (Ser473). Samples from 3 patients were tested. For 2 of 3 patients, samples showed a sustained rise in phospho-AKT following Hu1D10 treatment. Data shown here are representative of these 2.