α₄β₇⁺ and α₄β₇^– selected T cells do not differ in CD62L expression and alloreactive proliferation and cytokine secretion. (A) B10.BR splenic T cells were selected by magnetic separation, stained with anti-α₄β₇ antibodies, and separated in α₄β₇⁺ (filled curve) and α₄β₇^– (bold line) populations by flow cytometric–assisted cell sorting. These populations were again analyzed for CD4, CD8, and CD62L expression after sorting. (B) α₄β₇⁺ and α₄β₇^– sorted donor B10.BR T cells were labeled with CFSE and transferred into irradiated (750 cGy) CBA recipients, and donor splenic T cells from these recipients were analyzed after 40 hours. The number of dividing CD4⁺ or CD8⁺ CFSE-labeled donor T cells is indicated. (C) α₄β₇⁺ and α₄β₇^– sorted B10.BR T cells and B10.BR TCD-BM were transferred into irradiated (750 cGy) CBA recipients, and 3 days later splenocytes from these recipients were incubated for 5 hours with PMA and Ionomycine. Brefeldin was added during the last 3 hours of this incubation. Donor (Ly9.1^–) CD4⁺ or CD8⁺ T cells were analyzed for their intracellular IFN-γ expression. (D) B6 splenic T cells were labeled with CFSE and transferred into irradiated (750 cGy) syngeneic B6.Ly5.1 or allogeneic C3FeB6F1 recipients. After 3 days the expression of α₄β₇ on CFSE-labeled donor T cells in the spleen was determined.