Figure 3.
Figure 3. Recipients of α4β7+ T cells have significantly higher numbers of α4β7+ donor T cells in their intestinal mucosa and mesenteric lymph nodes. CBA mice received transplants as described in Figure 2. Recipients of α4β7+ and α4β7– T cells were killed on days 14 and 22 after BMT. Infiltrating α4β7+ T cells of donor origin were determined by multicolor flow cytometry in multiple organs. Top panels represent averages of absolute cell numbers (± SE) of α4β7+ CD4+ donor cells in spleen (day +14, n = 8; day +22, n = 9), peripheral lymph nodes (PLNs; day +14, n = 4; day +22, n = 4), mesenteric lymph nodes (MLNs; day +14, n = 3-4; day +22, n = 3-5), small intestine (gut, day +14, n = 3-4; day +22, n = 4-5) and liver (day +14, n = 6-7; day +22, n = 8-9). Bottom panels represent averages of absolute cell numbers of α4β7+ CD8+ donor cells in the above mentioned organs. *Signifies statistically significant P values.

Recipients of α4β7+ T cells have significantly higher numbers of α4β7+ donor T cells in their intestinal mucosa and mesenteric lymph nodes. CBA mice received transplants as described in Figure 2. Recipients of α4β7+ and α4β7 T cells were killed on days 14 and 22 after BMT. Infiltrating α4β7+ T cells of donor origin were determined by multicolor flow cytometry in multiple organs. Top panels represent averages of absolute cell numbers (± SE) of α4β7+ CD4+ donor cells in spleen (day +14, n = 8; day +22, n = 9), peripheral lymph nodes (PLNs; day +14, n = 4; day +22, n = 4), mesenteric lymph nodes (MLNs; day +14, n = 3-4; day +22, n = 3-5), small intestine (gut, day +14, n = 3-4; day +22, n = 4-5) and liver (day +14, n = 6-7; day +22, n = 8-9). Bottom panels represent averages of absolute cell numbers of α4β7+ CD8+ donor cells in the above mentioned organs. *Signifies statistically significant P values.

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