ICAM-4 peptides inhibit HT1080 cell binding to ICAM-4Fc. Adhesion of HT1080 cells to native ICAM-4 in the presence of assay buffer (positive control), defined peptides or EDTA (ethylenediaminetetraacetic acid), along with HT1080 adhesion to a NCAM-negative control. The results are expressed as a percentage of control cells bound □ SD (n = 3); 100% was equal to 41% of input cells bound, ICAM-4Fc was coated at a concentration of 7.5 μg/mL, and peptides were used at 500 μM.