CCL21-mediated activation of Erk-1/2 and PKB in MoDCs. MoDCs were matured in the presence or absence of PGE2 and subsequently were stimulated with CCL21 (250 ng/mL) for the indicated time periods. Whole-cell lysates were separated on SDS-PAGE and transferred to nitrocellulose membranes. (A) Membranes were stained with an antibody against phosphorylated (Thr202/Tyr204) Erk-1/2 (p-Erk) or total Erk-1/2 (t-Erk). (B) Membranes were probed with an antibody against phosphorylated (S473) PKB (p-PKB) or total PKB (t-PKB). Results from 1 of 4 independent representative experiments are shown.