PLC pathway is involved in MoDC migration to CCL19, CCL21, and CXCL12. (A) MoDCs matured with sCD40L and PGE2 were analyzed in a migration assay in response to CCL21 in the absence (▨) or presence (▦) of 2 mM EGTA in the assay buffer. MoDCs matured with sCD40L and PGE2 were incubated with titrated concentrations of (B) the PLC inhibitor U73122, (C) the PKC activator PMA, or (D) the PKC inhibitor staurosporine for 30 minutes at 37°C before migration to CCL19 and CCL21was analyzed in a transwell chemotaxis assay. (E) Migration to CXCL12 of MoDCs matured in the presence or absence of PGE2. The PI3K inhibitor wortmannin was used at 100 nM, and U73122 was used at 10 μM. Results from representative experiments of at least 3 different MoDC preparations are shown. Error bars indicate SEM.