Chemokine-induced Ca²⁺ mobilization is required for the migration of MoDCs to CCL19 and CCL21. (A) sCD40L- and PGE2-stimulated MoDCs were incubated with graded doses of BAPTA-AM for 30 minutes at room temperature before migration to CCL19 and CCL21 was analyzed in a transwell chemotaxis assay. Error bars indicate SEM. (B) sCD40L- and PGE2-stimulated MoDCs were loaded with fluo-3-AM alone (top) or with BAPTA-AM (bottom). Chemokine-induced Ca²⁺ mobilization was analyzed by flow cytometry. Baseline was established for 30 seconds before CCL21 was added, as indicated by the arrow. Representative results obtained from MoDCs of a single donor are shown. Three independent experiments with MoDCs from different donors yielded similar results.