Single-color flow cytometric analysis of RESDECs and HUVECs. (A) RESDECs. (B) HUVECs. Appropriate isotype control is indicated by an open plot, and indicated antibody by filled plot. Antibodies were either directly FITC-conjugated, or unconjugated antibodies were followed by staining with an FITC-conjugated goat antimouse (GAM-FITC) secondary antibody, as indicated. In the last panels, VEGF binding was measured using biotinylated VEGF followed by avidin-FITC (black plot). Specificity for VEGF staining is demonstrated by blocking with an anti-VEGF antibody (gray plot).