RT-PCR analysis of RESDECs and HUVECs. Total cellular RNA was isolated from RESDECs and HUVECs. Sequence-specific primers for the indicated genes were used for PCR analysis. RESDECs express RNA for VWF, flk-1, and CD34 as indicated, though no expression of CD31 and VE-cadherin is detected. All 5 genes are expressed in HUVECs. To control for contaminating genomic DNA, reactions were done using cellular RNA with no reverse transcriptase and these reactions did not demonstrate bands for any of the primers in either cell type.