Quantitative RT-PCR and allelic expression analysis of T-ALL transcription factor oncogenes. Black bars indicate biallelic expression, and gray bars indicate monoallelic expression. The levels of expression used as cutoff for positivity are indicated.2 Numbers at the top of each bar indicate the percentage of blast cells in the samples analyzed. Numbers at the bottom of each panel correspond to the sample identification numbers in the table in the Supplemental Document. (A) TAL1 expression in TAL1+ T-ALL allelic informative cases. Samples with a TAL1-d variant, resulting from deletion of a 90-kb genomic DNA fragment adjacent to the TAL1 locus in chromosome band 1p32, are indicated. (B) HOX11 expression in HOX11 T-ALL allelic informative cases. Samples harboring the chromosomal translocation t(10; 14)(q24;q11) that induces activation of the HOX11 locus are indicated. (C) LMO2 expression in LMO2 T-ALL allelic informative cases. NA indicates not available.
