Figure 3.
Telomere length assay and Western blots. (A) Telomere length assay. Passage 2 cells were plated at 100 cells/cm2 for 5 days and then incubated in medium with and without 17% FCS for 28 days as in Figure 1. Day-0 cells were assayed before initial plating. The control and SD cells were allowed to recover in medium with FCS for 5 days. (B) Western blot for p53 and p21 with hMSCs from 3 different donors. The hMSCs were incubated with and without serum as for panel A. A human fibrosarcoma cell line, HT1080, was used as a positive control.
