t(4;14) translocation and FGFR3 overexpression in patient MM cells. (Ai) Myeloma cells were evaluated for abnormalities of chromosome 4 and 14; (Aii) chromosome 13 abnormalities were evaluated with 2 probes (D13S319 and Rb) in the 13q14 region, using fluorescent in situ hybridization (original magnification, × 400). cIg indicates cytoplasmic immunoglobulin. (B) Normalized expression values from gene chip Hu95av2 for FGFR3 gene transcripts in CD138⁺ and CD138^– cells from BM from the patient with MM and the healthy twin. MM cells and healthy twin PCs were immunostained with anti-FGFR3 and anti-kappa light chain Abs (inset; original magnification, × 100). (C) Normalized expression values from gene chip Hu95av2 for oncogenes v-fos,c-myc, and v-myc gene transcripts in CD138⁺ and CD138^– cells from BM from the patient with MM and the healthy twin.