Increased antiapoptotic gene expression in MM cells versus healthy twin PCs. (A) Normalized expression values from gene chip Hu95av2 for antiapoptotic genes dad-1, FLIP, and mcl-1 gene transcripts in CD138⁺ and CD138^– cells from BM from the patient with MM and the healthy twin. (B) Cell lysates were prepared from MM cells and normal twin PCs, resolved by 10% SDS-PAGE, transferred onto nitrocellulose membrane, and probed with anti–mcl-1 and anti-tubulin Abs. Blots were developed by enhanced chemiluminesence, and relative protein expression in patient MM cells compared with normal twin PCs was calculated using densitometry.