Telomerase expression in MM cells versus healthy twin PCs. (A) Normalized expression values from gene chip Hu95av2 for telomerase and other telomere-related genes in CD138⁺ and CD138^– cells from BM from the patient with MM and the healthy twin. (B) Telomerase activity was measured using the TRAPeze telomerase detection kit. Cell lysates from patient MM cells and normal twin PCs following PCR amplification were electrophoresed on 12% nondenaturing polyacrylamide gel and stained with SYBR green I. Telomerase activity is determined by the ratio of the entire telomerase ladder to that of the internal control using densitometric analysis.