Figure 7.
ETO and Bcl-6 are present at the promoters of endogenous Bcl-6 target genes in B cells. Chromatin immunoprecipitations were performed on Daudi cells for endogenous proteins with either rabbit N-terminal ETO antibody, rabbit polyclonal Bcl-6 antibody, or rabbit IgG control. ChIP products were PCR amplified using primers surrounding the Bcl-6 binding site on the cyclin D2, CD69, and MIP1α promoters. DNA input as positive control and H2O as negative control are also shown. A control PCR reaction with primers from within ORF of Bcl-6 was also performed as a negative control.