Degree of cytoskeletal attachment of FTSC-labeled GPA. ρ_e/ρ_c is plotted against the dimensionless projection length, L/R_P for the FIMD of FTSC-labeled GPA in erythroblasts (A), reticulocytes (B), and mature red cells (C). L is the projection length and R_p is the radius of the pipette. Data were fitted by straight lines to calculate γ_GPA of FTSC-labeled GPA in erythroblasts (Eb), reticulocytes, and mature red cells. γ_GPA of FTSC-labeled GPA decreased progressively during red cell differentiation and reticulocyte maturation (D).