American Society of Hematology

Figure 6.

$Figure 6. Mad1–/– p27Kip1–/– MPROs are refractory to C/EBPϵ-induced differentiation. (A) Wild-type and Mad1–/–p27Kip1–/– MPROs were infected with control (pBabe, pB) or C/EBPϵ-ER (ϵ-ER) retrovirus. Following selection in 2 μg/mL puromycin, cells were treated with 200 nM 4-OH-tamoxifen (Tam) and differentiation was assessed morphologically over 4 days. Original magnification, × 400. (B) Equivalent expression of C/EBPϵ-ER in cell lines was determined by Western blot analysis in both bulk culture and clonal cell lines using an anti-ERα antibody.$

Mad1^–/– p27Kip1^–/– MPROs are refractory to C/EBPϵ-induced differentiation. (A) Wild-type and Mad1^–/–p27^Kip1–/– MPROs were infected with control (pBabe, pB) or C/EBPϵ-ER (ϵ-ER) retrovirus. Following selection in 2 μg/mL puromycin, cells were treated with 200 nM 4-OH-tamoxifen (Tam) and differentiation was assessed morphologically over 4 days. Original magnification, × 400. (B) Equivalent expression of C/EBPϵ-ER in cell lines was determined by Western blot analysis in both bulk culture and clonal cell lines using an anti-ERα antibody.

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