Medium requirements for mMSCs. (A) mMSCs were plated at 5 or 50 cells per cm2 (5 c or 50 c, respectively) and counted after 12 days of culture. (B) t-CFU assays were performed after the 12 days' incubation in the medium indicated. (C) t-CFU assays and sc-CFU assays were performed on Bl/6 mMSCs expanded in the different media. (D) Serum requirements (HS:FCS = 1:1) were determined for the 5 strains of mMSCs. The mMSCs were plated at 5 or 50 cells per cm2 (5 c or 50 c, respectively) and counted after 12 days. (E) t-CFU assays after the 12-day expansion in varying serum concentrations. (F) t-CFU and sc-CFU assays performed with mMSCs from Bl/6 mice. All strains were passage 7, except GFPtg, which was passage 4. Data are expressed as the mean ± SE, n = 3 to 5.
