Major and minor erythrocyte membrane proteins partition into DRM rafts to varying degrees. Uninfected erythrocyte membranes were extracted in 1% Tx-100 and subjected to sucrose density gradient centrifugation as described in “Materials and methods.” Fractions 1 to 6 were acetone precipitated and separated by SDS-PAGE. Blots were probed with appropriate primary and horseradish peroxidase–conjugated secondary antibodies and imaged as described. Results are shown for selected antibodies, oriented with the lightest, low-sucrose fraction on the left and the heaviest, sucrose-rich loading zone on the right. Fraction 2 contains DRMs. Adobe Photoshop (Adobe, San Jose, CA) was used to adjust brightness and contrast. EGFR indicates epidermal growth factor receptor; GPA, glycophorin A.