Immunocytochemical staining for leptin before and after induction of adipocyte differentiation of MSCs. Representative immunocytochemistry staining results for leptin expression in MSCs. (A) MSC monolayers before adipocyte differentiation culture and (B) after 14 days of adipocyte differentiation culture (original magnification, × 200). (C) Trypsinized MSC before adipocyte differentiation culture and (D) after 14 days of adipocyte differentiation culture (original magnification, × 400). After adipocyte differentiation, staining for leptin was more intense in cultured MSCs.