Effect of PDE3A inhibition by JF959602 on platelet accumulation into thrombi. Postinfusion thrombi and the matching preinfusion thrombi constituting a pair were compared for statistical analysis using the Wilcoxon rank sum test (*P < .05, **P < .01). (A) Platelet accumulation into thrombus before and after infusion of JF959602 of a representative experiment is shown. The corresponding fluorescence microscopy images at different time points following laser injury are shown above and below the graph. The infusion of JF959602 led to a significant inhibition of platelet accumulation into thrombus. (B) In the presence of 0.6 mg/kg (P < .05) and 1.2 mg/kg (P < .05) JF959602, a statistically significant decrease in the median of maximum platelet accumulation compared with the absence of JF959602 was observed. The numbers in parentheses indicate the number of thrombi in each data set. (C) A dose-dependent inhibition of the median stabilized platelet accumulation was also observed in the presence of 0.6 mg/kg (P < .05) and 1.2 mg/kg (P < .01) JF959602. The numbers in parentheses indicate the number of thrombi represented by each data set.