Responses of TCRBV 17⁺ CD4⁺ T lymphocytes from LTNP-100149 to HIV-1 p24 peptide no. 42. (A) Flow cytometry histograms, gated on CD3⁺CD4⁺ TCRBV 17⁺ and TCRBV1⁺ lymphocytes, respectively, showing proliferative responses of CFSE-labeled CD4⁺ T cells in response to p24, peptide no. 42, and peptide no. 106. (B) Flow cytometry histograms, gated on CD3⁺CD4⁺ TCRBV 17⁺ and TCRBV1⁺ lymphocytes, respectively, showing IFN-γ responses to p24, peptide no. 42, and peptide no. 106 in ICC assays. (C) Flow cytometry histograms, gated on CD3⁺CD4⁺ lymphocytes, showing cell surface expression of CD69, CD25, and CD40L after incubation of whole blood for 48 hours with peptide no. 42. (D) Flow cytometry histograms, gated on CD3⁺CD4⁺ lymphocytes, showing simultaneous intracellular production of IFN-γ, IL-2, and CD40L in ICC assays. Results are representative of at least 3 independent experiments, on different visits, over more than 1 year.