Molecular characterization of PML Mut 1 and Mut 2 variants. (A) Western blot analysis of wt PML, Mut 1, and Mut 2 overexpressed in PML^-/- mouse fibroblasts. (B) Immunofluorescence analysis reveals that PML Mut 1 and Mut 2 localize aberrantly in the cytoplasm as indicated by the arrows. Original magnification × 60.