Figure 5.
Expression of ATF-4 in primary human breast cancer tumors correlates with that of GADD153, and tumor-section staining indicates ATF-4 expression near necrotic areas. (A) Extracts made from primary malignant human breast tumors were analyzed by immunoblot for ATF-4 and GADD153 protein levels. MDA-MB 435 cells incubated for 16 hours in normoxia or anoxia were used as control. (B) Paired malignant tumor (T)/normal (n) tissue extracts were analyzed using immunoblot for ATF-4 expression. (C) After dewaxing and rehydration, 5-μm sections of metastatic breast tumors were analyzed for ATF-4 expression by immunostaining. Original magnification, × 120.