Profile of IL-7Rα expression in AKR/J mice. Expression of IL-7Rα was examined at level of surface protein by FACS staining (A,D) and at level of mRNA transcripts by RT-PCR analysis (C). Cells were isolated from thymus (i), lymph nodes (ii), spleen (iii), or BM (iv) of 2-month old AKR/J or control B10.BR mice. (A) FACS analysis was performed in total cells using biotin-conjugated anti–IL-7Rα antibody in combination of a cocktail of PE (anti-CD4, anti-CD8, anti-TCRαβ)–conjugated antibodies (i), or in combination with anti-B220–PE antibody (ii-iv). Numbers shown in FACS profiles denote percentages of cells in each quadrant. Table (B) represents values of the mean fluorescence intensity (MFI) of IL-7Rα expression ± SE in indicated cell population from 4 independent experiments with n = 5 mice for each experiment. All values in bold represent significantly increased MFI (P < .05) on AKR/J cells compared with control B10.BR cells. (C) RT-PCR analysis to investigate expression of IL-7Rα transcripts was performed by using sorted TN (CD4^–CD8^–TCRαβ^–) thymocytes (i), B (B220⁺) lymphocytes (ii-iv), or T (TCRαβ⁺) lymphocytes (iii-iv) isolated from 2-month-old B10.BR (lane 1) and AKR/J (lane 2) mice. (D) Histograms represent intensity of γc expression on gated TN thymocytes (i) or B lymphocytes from lymph nodes (ii), spleen (iii), or BM (iii) isolated from 2-month-old B10.BR (dashed line) or AKR/J (solid line) mice. Results (C-D) are representative of 3 independent experiments.