Putative HOXA9 gene target expression in other cell types. (A) JUNB and EPS8 expression levels are modulated by HOXA9 in NIH3T3 fibroblasts. HOXA9, JUNB, and EPS8 expression levels were measured in RNA from parental cells and stably transduced HOXA9 overexpressing cells by real-time PCR. (B) Confirmation of converse changes of EPS8 and JUNB in HOXA9-deficient murine marrow cells. Marrow cells were isolated from adult HOXA9^–/– mice and wild-type (control) littermates and enriched for Lin^– Sca⁺ primitive cells. HOXA9, JUNB, and EPS8 levels were assessed by real-time PCR. EPS8 levels are multiplied by 1000 for scaling purposes.