Effect of APC on binding of STAT6 and NF-κB oligonucleotides to nuclear proteins. The binding capacity of both NF-κB (A) and STAT6 (B) oligonucleotides to nuclear proteins was significantly increased in the OVA + SAL group compared with the saline group. However, the binding activity of both oligonucleotides was significantly decreased in mice of the OVA + APC group compared with the OVA + SAL animals. No binding activity of STAT6 or NF-κB oligonucleotides was observed in the APC group. The figure shows the representative results of experiments performed 3 times from 3 separate experiments. The effect of APC on STAT6 was also evaluated in cell lines known to have an intact JAK/STAT pathway. APC inhibited markedly the binding of STAT6 oligonucleotides to nuclear proteins from both U-937 (C) and HeLa cells (D). The figure is the representative of 3 experiments carried out using 4 different samples.