Forced expression of C/EBPα induces differentiation of 32D cells expressing FLT3/ITD. The 32D/α-ER/FLT3/ITD cells were incubated with 1 μM estradiol for 2 days. (A) Cytospins were prepared on days 0 and 2. The morphologic features of the cells were visualized by means of Wright-Giemsa staining followed by light microscopy. Original magnification × 40. (B) Total cellular RNA was prepared from 1 × 10⁷ cells 0, 8, 24, and 48 hours after incubation with estradiol. RNA (10 μg from each sample) was then subjected to sequential Northern blotting with MPO, lysozyme, lactoferrin, and actin cDNA probes.