Dok-2 tyrosine phosphorylation induced by TRAP. (A) 2-DE gel representative image showing up-regulation of a Dok-2 protein feature upon TRAP stimulation. (B) Whole protein lysate and phosphotyrosine immunoprecipitated proteins or (C) Dok-2 immunoprecipitated proteins were separated by SDS-PAGE and immunoblotted using 4G10 antiphosphotyrosine or anti–Dok-2 antibodies. B indicates basal; T, TRAP (5 μM, 30 s); IP, immunoprecipitation; and IB, immunoblot. Images represent at least 3 independent experiments.