Figure 5.
Figure 5. IL-7 down-regulates p27kip1 expression and induces hyperphosphorylation of Rb in TAIL7 cells. (A) Lysates of TAIL7 cells cultured for 96 hours were resolved by 10% SDS-PAGE and immunoblotted with a p27kip1-specific antibody. The membrane was stripped and reprobed for ZAP-70 to demonstrate equal protein loading. (B) Lysates were analyzed by 6% SDS-PAGE and immunoblotted for Rb. The antibody recognizes both hypophosphorylated (pRb) and hyperphosphorylated (ppRb) forms of Rb. Representative results of 3 independent experiments are shown.

IL-7 down-regulates p27kip1 expression and induces hyperphosphorylation of Rb in TAIL7 cells. (A) Lysates of TAIL7 cells cultured for 96 hours were resolved by 10% SDS-PAGE and immunoblotted with a p27kip1-specific antibody. The membrane was stripped and reprobed for ZAP-70 to demonstrate equal protein loading. (B) Lysates were analyzed by 6% SDS-PAGE and immunoblotted for Rb. The antibody recognizes both hypophosphorylated (pRb) and hyperphosphorylated (ppRb) forms of Rb. Representative results of 3 independent experiments are shown.

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