JAK3 inhibitor WHI-P131 abrogates IL-7–induced proliferation and viability in TAIL7 cells. TAIL7 cells were cultured for 72 hours in medium alone, in the presence of IL-7, or with IL-7 plus 100 μM WHI-P131 (J3In.). (A) Viability was assessed by Annexin V–FITC/PI staining and FACS analysis. (B) Proliferation was examined by 3H-Thymidine incorporation and is expressed as mean ± SD of triplicates. (C) Effects of rapamycin (Rapa; 10 nM), dexamethasone (Dexa; 100 nM), and doxorubicin (Doxo; 100 nM) on IL-7–mediated viability of TAIL7 cells. Cultures were performed in duplicates for 96 hours and results are expressed as mean ± SD. (D) For each condition, TAIL7 cell viability was normalized, comparing to the IL-7–mediated effect (100%). Results at 72 hours and 96 hours of drug treatment are shown for a representative experiment.
