CHO cells expressing P-selectin adhered to immobilized ULVWF under static condition. (A) CHO cells stably expressing human P-selectin (CHO-P), as determined by flow cytometry with PBS buffer, and cells that remained adhered were counted (B). Cell adhesion was also measured in the presence of 0.5 μM EDTA (B, bar = 100 μm). CHO-P cells specifically adhered to ULVWF but not to BSA surface, and adhesion was blocked by treating cells with EDTA or a polyclonal anti–P-selectin antibody (C, the Student t test, n = 4).