Ferritin L traverses the classical secretory route. (A) BFA inhibits the secretion of apolipoprotein B (apoB), which is secreted via the classical secretory route. AdX-FL–infected HepG2 cells were left untreated (-) or treated with 1 μg/mL BFA (+). The cell lysates (labeled C) and medium (M) were collected, and one aliquot of each was subjected to immunoprecipitation with polyclonal antibodies against apolipoprotein B. (B) BFA inhibits the secretion of ferritin L. Aliquots of the cell lysate (labeled C) and medium (M) from panel A were immunoprecipitated with polyclonal antibodies against ferritin.