FLT3-TKD mutants differ significantly in their sensitivity to the growth inhibitory activity of the FLT3 PTK inhibitor SU5614. (A) Dose-response curves of the inhibitory activity of SU5614 in Ba/F3 FLT3-ITD and FLT3-TKD cells after 72 hours of incubation. Ba/F3 cells expressing different FLT3 constructs were seeded at a density of 4 × 104 cells/mL in the absence or presence of different concentrations of SU5614. Viable cells were counted after 72 hours by trypan blue exclusion. The growth of cells that were incubated without inhibitor was defined as 100%. Values represent means and standard errors from 3 independent experiments. (B) Sensitivity of FLT3-ITD and TKD mutants to SU5614. Ba/F3 cells were seeded at a density of 4 × 104 cells/mL in the presence of 1 μM SU5614 and counted after 72 hours. Values represent means and standard errors from 3 independent experiments.
