SU5614 induces apoptosis in FLT3-ITD and FLT3-D835Q/V/Y cells but not in FLT3-D835H cells. (A-B) Ba/F3 cells transduced with the FLT3-ITD, FLT3-D835Y, or FLT3-D835H constructs were incubated with different concentrations of SU5614 for 24 hours and were analyzed by flow cytometry after staining with annexin V-PE and 7-AAD. Representative dot plots from 1 of 3 independently performed experiments are shown. Values represent means and standard deviations from 3 independent experiments. The numbers in dot-plot quadrants (B) show the percentages of annexin V⁺/7-AAD^- (lower right quadrants) and annexin V⁺/7-AAD⁺ (upper right quadrants).