Generation of TTR-hepc2transgenic mice. (A) Schematic representation of the TTR-hepc2 construct. The murine hepc2 cDNA was introduced between the transthyretin sequences (consisting of the 3 kb of the mouse TTR regulatory regions 5′ to the cap site, the first exon, first intron, and most of the second exon) and the SV40 small-T poly(A) signal sequence.¹⁷  (B) Southern blot analysis of tail DNA from transgenic founders. Genomic DNA was digested by BamHI and hybridized with the TTR probe shown in panel A. Two bands of the expected size, 5.3 kbp and 4.7 kbp, representing the endogenous TTR gene (End) and the transgene (Tg), respectively, were detected.