Figure 1.
Construction and expression analysis of the Bcl-XL transgenic mouse. (A) Diagram of the 3′KE/Bcl-XL transgenic construct. (B) Southern blot analysis of tail-snip DNA to identify founders. (C) RT-PCR and Western blot analysis to determine tissue-specific transgene expression. (D) RT-PCR to detect transcripts in FACS-sorted lymphocytes (S indicates spleen; M, marrow).