Expression of Eμ- and VavP-Bcl2 transgenes in lymphoid cells. (A) Flow cytometric analysis of transgenic Bcl2 detected by immunofluorescence staining with Bcl2-100 antibody in B220⁺ spleen cells. Cells from C57BL/6 mice served as the negative control. (B) Transgenic Bcl2 in B220⁺ B cells and Thy-1⁺ CD4⁺ and CD8⁺ T cells from the spleen. C57BL/6 (n = 10), Eμ-Bcl2 22 (n = 5), and Eμ-Bcl2 36 (n = 5) mice were compared with pooled data from 3 strains of VavP-Bcl2 mice (total n = 16). Fluorescence intensity values were normalized for nonspecific staining using nontransgenic C57BL/6 controls.