Analysis of skin-derived DC populations in cutaneous LNs from ICSBP^-/- mice. (A) Skin-draining lymph nodes from ICSBP^-/- and WT mice were enriched on Nycodenz and double-stained for CD11c and CD40 expression. Dot plots represent DC populations identified by CD11c and CD40 expression. Values are calculated on the population of LN suspensions from at least 5 mice. (B) ICSBP^-/- and WT animals were given BrdU in drinking water for 15 days. At the indicated time points, cutaneous LNs were removed and triple-stained for determination of BrdU uptake in the CD40^hi CD11c⁺ DC subset. Data are representative of 2 separate experiments.