Phenotype of BM-derived DCs from ICSBP^-/- mice. BM from ICSBP KO and control WT animals was cultured for 7 days in presence of GM-CSF. (A) BM DCs were double-stained for CD11c and a panel of antibodies specific for the indicated markers. Data represent the expression of the indicated markers in the population gated on forward-side scatter properties and on CD11c positivity. Gray-filled graphs represent the specific staining for the indicated markers, while broken lines are the isotype-matched controls. (B) RT-PCR analysis of IL-12p40 expression at various times of BM culture. Day 0 represents the time of bone marrow extraction. Representative data of 1 of 3 separate experiments are shown.