Figure 3.
RT-PCR analysis of EPDCs and HUVECs. Expression of CD133, CD144, KDR, Tie-2, Flt-1, and GAPDH mRNA was determined with a 32P-labeled internal probe at days 14, 28, and 40 of culture for EPDCs and at day 21 of culture for HUVECs. RT- was the control realized without reverse transcription before PCR. D indicates day.