Figure 5.
Effect of VEGF, FGF-2, or PlGF on EPDC and HUVEC proliferation, survival, and mobility. Cell proliferation was analyzed (A) in the presence of 2.5% of FBS or (B) under serum starvation conditions. (C) The percentage of living cells compared with the control (EGM2-MV medium depleted in FBS, FGF-2, and VEGF) was estimated by annexin V/PI staining. The annexin V-/PI- population was representative of living cells. (D) Effect of 10 ng/mL FGF-2, VEGF, or PlGF on EPDC and HUVEC mobility compared with control conditions (EGM2-MV medium containing 0.1% BSA, depleted in FBS, FGF-2, and VEGF).