Purification of HSPC populations from FB and MPB. (A) Lineage-depleted population from several fetal blood (n = 10) and MPB (n = 6) samples were pooled separately and stained with CD34 antibody conjugated to APC and CD38 antibody conjugated to PE. Using a stringent sorting gate (R1), the CD34⁺CD38^- population in FB (i) and MPB (iii) were sorted and reanalysis of the sorted populations ensured the high purity of FB Lin^-CD34⁺CD38^- (ii) and MPB Lin^-CD34⁺CD38^- (iv) cells. (B) Amplification of β-actin and β-glucoronidase to verify the integrity and maintenance of the abundant of primary transcripts after cDNA amplification in FB Lin^-CD34⁺CD38^- and MPB Lin^-CD34⁺CD38^-. Different concentrations (7 ng, 0.7 ng, and 0.07 ng) of amplified cDNA from FB Lin^-CD34⁺CD38^- and MPB Lin^-CD34⁺CD38^- were used as the starting material in reverse transcriptase–PCR (RT-PCR) for the amplification of β-actin, as one of the highly expressed housekeeping genes, and β-glucoronidase, as one of the genes present as a single transcript per cell.