Quantitative measurement of selected differentially regulated genes in FB-HSPCs versus MPB-HSPCs and FB-HPCs. (A) The cDNA of pooled FB-HSPCs and MPB-HSPCs was used in Q-PCR to evaluate the expression level of some of the differentially regulated genes in the group of transcription factors, cell cycle regulators, and signal transduction genes. Q-PCR reactions were performed in duplicate and GAPDH was used as an internal control in Q-PCR reactions, and the relative expression of these genes in FB-HSPCs versus MPB-HSPCs was calculated by 2^-ΔΔCt equation (“Materials and methods”). Error bars indicate standard errors. (B) The same methodology was used to calculate the relative expression of the selected transcripts in FB-HSPCs versus FB-HPCs. ^*Lack of expression in FB-HPCs. (C) Amplification plot of FB248 as the transcript exclusively expressed in HSPC populations but not in FB-HPC. GAPDH amplification is shown as an internal control for Q-PCR reaction. However, FB248 amplification demonstrates the presence of amplicon in FB-HSPCs (Ct = 28.5) and MPB-HSPCs (Ct = 31.0) but not in FB-HPCs.