Modulation of the transcriptional activity of EBF by EHZF. 293 cells were transfected with a β-galactosidase expression plasmid as an internal control, the luciferase reporter plasmids human pB29-Luc (A) and pλ5-Luc (B), and either void vector (p3xFlagCMV7.1) or p3xFlagCMV7.1-EHZF (2 μg), pCDNA3.EBF-6xmyc(2 μg), pCDNA3.EBF-6xmyc (2 μg) + p3xFlagCMV7.1-EHZF (1 μg), pCDNA3.EBF-6xmyc (2 μg) + p3xFlagCMV7.1-EHZF (2 μg) or pCDNA3.EBF-6xmyc (2 μg) + pFlagCS2-OAZ(KIAA0760-L) (2 μg). Extracts were prepared 48 hours later, assayed for β-galactosidase, normalized, and assayed for luciferase activity. The results shown are the average of triplicates from 3 independent experiments. Error bars indicate standard deviations.