Figure 4.
Dominant-negative AP-1 inhibits Sp1 enhancement of MEKK-1—stimulated MRE activation. (MRE)2/Luc was transfected into VSMCs with pCDNA3.1 blank vector or MEKK-1* in the presence of pCMV empty vector, Sp1, A-Fos, or Sp1 and A-Fos. Cells were lysed 1 day after the start of transfection and luciferase activities were assayed. Renilla luciferase activities of cotransfected pRL-TK were used to normalize transfection efficiency. All transfections were performed in triplicate, and the bar graph shows results from 3 separate experiments.
