MRE mediates Ang II—stimulated PAI-1 expression. VSMCs infected with adenoviruses containing PAI-1(-764)/Luc, PAI-1(-88)/Luc, PAI-1(-50)/Luc, as well as Luc without PAI-1 promoter (control), were starved and stimulated by Ang II for 3 hours with or without 50 μM PD98059 (PD) pretreatment. Northern blot analysis was performed by using a probe for luciferase and PAI-1, respectively. Representative blots of luciferase and PAI-1 mRNA expression and a bar graph quantitation of luciferase mRNA levels normalized to green fluorescence protein (GFP) mRNA of unstimulated cells from 3 separate experiments are shown. ^*P < .05 and ^**P < .001 by ANOVA.